VRK2 monoclonal antibody (M01), clone 3B10
产品名称： VRK2 monoclonal antibody (M01), clone 3B10
英文名称： VRK2 monoclonal antibody (M01), clone 3B10
产品编号： H00007444-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant VRK2.
- Immunogen:
- VRK2 (AAH27854, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- VQTAKTNLLDELPQSVLKWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINVHTPNSQKVDSQKAATKQVNKAHNRLI
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- VRK2 monoclonal antibody (M01), clone 3B10. Western Blot analysis of VRK2 expression in U-2 OS ( Cat # L022V1 ).
- Protocol Download
- Western Blot (Cell lysate)
- VRK2 monoclonal antibody (M01), clone 3B10 Western Blot analysis of VRK2 expression in K-562 ( Cat # L009V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody (M01), clone 3B10.
Lane 1: VRK2 transfected lysate(58.1 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged VRK2 is approximately 1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi ( Cat # H00007444-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody (M01) clone 3B10 (Cat # H00007444-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 7444
- GeneBank Accession#:
- BC027854
- Protein Accession#:
- AAH27854
- Gene Name:
- VRK2
- Gene Alias:
- -
- Gene Description:
- vaccinia related kinase 2
- Omim ID:
- 602169
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
- Other Designations:
- vaccinia virus B1R-related kinase 2,vaccinia-related kinase-2
- Related Disease